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ANTIOXIDANT PROPERTIES EVALUATION OF TOTAL PLANT EXTRACT FROM SATUREJA CAERULEA HERBA IN EXPERIMENTALLY INDUCED SKIN BURNS IN WISTAR RATS

Andrei Dan Branza, Victoria Badea, Gabriela Gegiu, Mircea Grigorian, Stelian Schiopu, Marius Daniel Radu

Abstract

Background Satureja caerulea is an endemic aromatic plant of the Satureja genus, Lamiaceae family, related with species of Thymus and Rosemary, used in ethnopharmacological practice for its curative effects. This study aims to assess the antioxidant properties of hydroalcoholic total plant extract (40%) of Satureja caerulea herba by assessing the tisular level of malonildialdehyde measured in the skin graft of Wistar rats. Material and methods The plant material was harvested from Dobrogea area in August 2013. Eighteen male Wistar rats were divided into three groups: a control reference group (AM) and two experimental groups (AV; AEx). We induced thermal burns on the animals’ skin on a 1.5 cm radius circle area. Thereafter, for five days we administered medical grease over the burn wound of the AV experimental group and total plant extract of Satureja10% included in medical grease over the wounds of the AEx experimental group. At the end of the experiment, we took samples under anesthesia, from the area of skin burns and tissue levels of malonildialdehyde was determined as described by Drapper and Hadley (1990). The method consists in the reaction of malonildialdehyde, produced by peroxidation of membrane lipids, with thiobarbituric acid, in trichloroacetic acid presence. The pale pink complex formed it is read spectrophotometrically at 532nm. In parallel we determine the concentration of protein in the sample and the concentration of malonildialdehyde is related to mg protein. Results Malonildialdehyde is an indicator of membrane lipid peroxidation, tissue levels of malonildialdehyde increase only if the processes occurring massive lipid peroxidation of cell membranes, resulting in cytolysis event. In our experimental model malonildialdehyde values expressed in nmol / mg protein are in reference control group (AM) 1.38±0.16, for the experimental group (AV) 0.50±0.05 and 0.25 ± 0.07 for the experimental group (AEX). Conclusions The decrease of malonildialdehyde in dynamic indicates that in our experimental model, after five days of topical skin application of phitoproduct, an adaptation of antioxidant defense system appears and a limitation of negative cytological effects associated with oxidative stress phenomenon. Our results can be the starting point in achieving a therapeutic product with effect in burned wounds.

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DOI resolver: doi.org/10.5593/sgemsocial2014/B12/S2.010

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