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THE INFLUENCE OF DEMINERALIZING FACTORS ON HUMAN ENAMEL MICROHARDNESS MEASUREMENTS
Abstract
Background: Knowing the real values of enamel mechanical properties, both at macro and microscopic scale, it is highly significant to select those compatible dental materials, in terms of behavior, as close as possible to the natural structures. The aim of the study was to assess human enamel microhardness both in normal enamel samples (control) and in demineralized enamel samples, using the Vickers microhardness test, and also to analyze the effect of indentation loads (100gf, 200gf, 300gf) applied for 10s on the hardness values derived. Methods: Enamel hardness was initially measured (in 7 samples) and again after 192 hours (8 days), during which time other 7 samples were immersed in 5ml of Coca-Cola and 7 samples in 5ml of Cherry Prigat (the solvent being refreshed every 24 hours). The indentations were carefully performed (10 for each sample) and the mark left was checked for symmetry. Data gathered during the experiment were processed with IBM SPSS Statistics 20. Results: The Vickers Hardness Number (VHN) values for enamel control samples are in the range of 320-390 Kgf/mm2, while for demineralized enamel samples, the VHN values are in the range of 210-340 Kgf/mm2, these values being in keeping with literature studies. One-Way ANOVA analysis of variance together with Post Hoc Tests - Multiple Comparisons (Bonferoni/ Tamhane), revealed significant statistical differences in VHN of enamel (p < 0.001), among the samples under research (Control, immersed in Coca-Cola and in Cherry Prigat) for a certain indentation load (100gf, 200gf, 300gf), applied for 10s indentation time. Conclusions: The highest mean values of enamel hardness are identified in the control group, followed by the samples immersed in Coca-Cola and, last, in the samples immersed in Cherry Prigat.
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