Scholarly record
RESEARCH ABOUT HUMAN BETA-3 DEFENSINS AND INTERLEUKIN-1BETA IN PERI-IMPLANTITIS MONITORING AND DIAGNOSIS
Abstract
Aim: The purpose of the present study was to compare the levels of Interleukin-1beta (IL-1beta) and Human beta 3 Defensin (hBD-3) in peri-implant crevicular fluid (PICF) and to assess if there is a correlation between this two parameters and the orodental clinical status in peri-implantitis. Microbial colonization of dental implants and infection of the peri-implant tissues can cause peri-implant bone destruction and may lead to implant failure. Inflammatory mediators of the host, such as IL-1beta, and antimicrobial peptides, as hBD-3, from saliva and crevicular fluid, produced as a response to bacterial infection, have an insufficient known implication in peri-implantitis. Methods: The study comprised 24 patients with dental implant (16 with 3 implant/subject, 8 with 5 implants/subject) which were clinically orodental evaluated in a private dental clinic from Constantza, Romania during 2016. They were examined after 1 and 4 week since dental implant installation. The PICF samples were collected by using sterile paper points which were introduced into each peri-implant site for 30 seconds. After clinical exam we quantified the hBD-3 and Il-1beta in PICF by using ELISA method (Alfa Diagnostic, Salimetrics, USA). The results were statistically analyzed by MedCalc 11.0 and for a p<0.05 was considered to have statistical significance. The study was approved by the Ethics Committee for medical research from Ovidius University of Constanta and the signed agreement from each subject was obtained. Results: After the clinical examination we found: 9 healthy implants (5 males, 55,6% and 4 females, 44,4%; mean age 47.7±5.46 years; PD?3, BOP=0) and 15 patients with peri-implantitis (9 males, 60% and 6 females, 40%; mean age 53.3±2.70 year; PD?3, BOP=1). The results show that there are high statistically significant differences between the level of IL-1beta (p<0.0001) and hBD-3 in healthy implant sites versus peri-implantitis patients (p<0.0001). Also, there is a high correlation between the level of IL-1beta and the peri-implantar sulcus depth at peri-implantitis patients (r=0.799, p=0.0003 at 1 week and r=0.765, p=0.0009 at 4 weeks); we found correlation between the levels of hBD-3 and the peri-implantar sulcus depth at our patients (r=-0.965, p<0.0001 at 1 week and r=0.700, p=0.03 at 4 weeks). Conclusions: The relationship between the oro-dental status, hBD-3 and IL-1beta suggests that the levels of these two parameters can be used as biomarkers in the diagnosis and monitoring of the peri-implantitis.
Publication Impact Profile
Publication details
References13
Misch E.C., Dental Implant Proshetics, 2nd Edition, St. Louis: Mosby Elsevier, 2015, pp 1-1001.
American Academy of Periodontology Report: Peri-Implant Mucositis and Peri-Implantitis: A Current Understanding of Their Diagnoses and Clinical Implications, Journal of Periodontology, vol. 84:4, pp 436-443, 2013.
Mombelli A., Muller N., Cionca N., The epidemiology of peri-implantitis, Clinical Oral Implants Research, 23:Suppl 6, pp 67–76, 2012.
Bhagawati S.B., Bhagawati B.T., Mittal S., Role of Defensins in Oral Diseases, Journal of Indian Academy of Oral Medicine and Radiology, vol. 24:2, pp 142-145, 2012.
Dale B.A., Fredericks L.P., Antimicrobial Peptides on the Oral Environment: Expression and Function in Health and Disease, Current issues in molecular biology, vol. 7:2, pp 119-133, 2005.
Gualini F., Berglundh T., Immunohistochemical characteristics of inflammatory lesions at implants, J Clin Periodontol., vol. 30(1), pp 14-18, 2003.
McPetersen R.A., Pincus M.R., Immunology and Immunopathology. In Henry’s Clinical Diagnosis and Management by Laboratory Methods – Sauders Elsevier 21st Edition 2007, pp 862.
Rich R.R., Fleisher T.A., Cytokines and Cytokines receptors. In Clinical Immunology, Principles and Practice – Mosby Elsevier 3rd Edition 2008, pp 143-165.
Guncu G.N., Akman A.A., Gunday S., Yamalik N., Berker E., Effect of inflammation on cytokine levels and bone remodelling markers in peri-implant sulcus fluid: A preliminary report, Cytokine, vol. 59, pp 313-316, 2012.
https://www.salimetrics.com/assay-kit/interleukin-1-il-1-salivary-immunoassay-kit-elisa-eia, cited 16.06.2017.
http://www.4adi.com/commerce/ccp9361-human-beta-defensin-3-28bd-329-elisa-kit--96-tests--elisa-kit-100-260-bd3.htm, cited 28.06.2017.
Kuula H., Salo T., Pirilä E., Hagströme J., Luomanen M., Gutierrez-Fernandez A., Romanos G.E., Sorsa T., Human β-defensin-1 and -2 and matrix metalloproteinase-25 and -26 expression in chronic and aggressive periodontitis and in peri-implantitis, Archives of Oral Biology, vol. 53(2), pp 175–186, 2008.
Ata-Ali J., Flchy-Fernandez A.J., Alegre-Domingo T., Ata-Ali F., Palacio J., Penarrocha-Diago M., Clinical, microbiological, and immunological aspects of healthy versus peri-implantitis tissue in full arch reconstruction patients: a prospective cross-sectional study, BMC Oral Health, vol. 1, pp 15-43, 2015.
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.
